Coding

Part:BBa_K3398004

Designed by: MingXiao Wei   Group: iGEM20_ZJU-China   (2020-10-17)


mamC_ZZ fusion protein with GST tag

This part is encodes a fusion protein of mamC and ZZ with a GST tag at the N- teminus for separation from other proteins at the purification and a thrombin site between the fusion protein and tag for cleavage after purification. For detailed information about the fusion protein of mamC and ZZ, we have submitted it as BBa_K3398003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85
    Illegal SapI.rc site found at 729


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Categories
Parameters
None