Composite

Part:BBa_K3396018:Design

Designed by: Huiying Liu   Group: iGEM20_NUDT_CHINA   (2020-10-27)


CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


Design Notes

FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.


Source

Human

References