Composite

Part:BBa_K3395005

Designed by: YONG JI   Group: iGEM20_RDFZ-China   (2020-10-23)


pTac-musTPH1

IGEM RDFZ_2020 team constructs pLacIQ promoter, musTPH1 generator, and terminator together to form understeam effector, the downstream effector will express when IPTG is present because the pLacIQ promoter will be repress when upstream LacI is express. This system will present the signal showing the enzymatic activity of the TPH1. To test the signal, you need to add IPTG first and growing overnight culture. By this, you can get enough enzymes in the solution. Then, we added tryptophan. Which is the rare material of 5-HTP. Because the concentration 5-HTP can influence the spectrum at excitation 300nm and emission 330nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
    Illegal XhoI site found at 241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 448


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Categories
Parameters
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