Tag

Part:BBa_K3394002:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)


Histidine tag (His-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design, we added a histidine tag (His-tag) at the N-terminus of our passenger proteins - either cellulase Endo5a or CBD (cellulose binding domain).



Source

Artificial sequence - synthetic construct

References

Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V. S., & Yazdani, S. S. (2011). Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Applied and environmental microbiology, 77(14), 4859-4866.