Coding

Part:BBa_K3394001:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)


Coding Sequence of CBD (Cellulose Binding Domain)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 37
    Illegal AgeI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.

Source

GenBank: CAA48312.1 (NCBI)

References

Szczupak, A., Aizik, D., Moraïs, S., Vazana, Y., Barak, Y., Bayer, E. A., & Alfonta, L. (2017). The electrosome: a surface-displayed enzymatic cascade in a biofuel cell’s anode and a high-density surface-displayed biocathodic enzyme. Nanomaterials, 7(7), 153.