Tag

Part:BBa_K3385043:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-12)


SigP_faeA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 44
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The long unpaired overhangs used were considered to be a problem when performing PCR amplification. Other methods like MoClo also have to be considered to ensure efficient modulation for future use.

Source

This signal peptide was obtained through DNA synthesis.

References

[1] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)

[2] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431