Composite

Part:BBa_K3384137:Design

Designed by: Yiqing Zhang   Group: iGEM20_NJTech_China   (2020-10-26)

pprm1 Ultra-GFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1157


Design Notes

Please visit to the design of the basic part BBa_K3384314 for the construction process of pprm1 Ultra. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.


Source

Saccharomyces cerevisiae