Composite

Part:BBa_K3384132

Designed by: Yiqing Zhang   Group: iGEM20_NJTech_China   (2020-10-20)

pfig1-GFP-CYC1 terminator

A pheromone-responsive reporter pathway in Saccharomyces cerevisiae used to characterize the activity of pFig1 BBa_I766558. As is shown in figure1, we quantitatively measured the activity of pFig1 at different pheromone concentrations.



NJTech China--pfig1.png


Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1644


[edit]
Categories
Parameters
chassisSaccharomyces cerevisiae