Part:BBa_K3380511:Design
No scaffold DIR2s-Apt aptamer under T7RNAP promoter (BBa_z0251) and T7 terminator (BBa_K3137010)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25
Design Notes
This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380511 construct can be assembled following a similar protocol (on the experience page of the BBA_K3380500 construct).
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase
Source
The parts were acquired as de novo synthesized sequences from Sigma-Aldrich.
References
Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230.
Tan, X., Constantin, T.P., Sloane, K.L., Waggoner, A.S., Bruchez, M.P. and Armitage, B.A., 2017. Fluoromodules consisting of a promiscuous RNA aptamer and red or blue fluorogenic cyanine dyes: selection, characterization, and bioimaging. Journal of the American Chemical Society, 139(26), pp.9001-9009.