Composite

Part:BBa_K3376011

Designed by: Li, Sheng-Fong   Group: iGEM20_Mingdao   (2020-10-06)


tpxp-KatG-Tr/pSB1C3

Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli Nissle strain (EcN) was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.

T--Mingdao--ww5.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 424
    Illegal BglII site found at 1359
    Illegal BglII site found at 1486
    Illegal BamHI site found at 1276
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None