Composite

Part:BBa_K3373014:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)


BSR_NucA-TEVsite_UVR8-NtTEV_COP1-CtTEV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1356
    Illegal EcoRI site found at 3476
    Illegal EcoRI site found at 6675
    Illegal PstI site found at 4323
    Illegal PstI site found at 7893
    Illegal PstI site found at 8070
    Illegal PstI site found at 8418
    Illegal PstI site found at 8433
    Illegal PstI site found at 9858
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1356
    Illegal EcoRI site found at 3476
    Illegal EcoRI site found at 6675
    Illegal NheI site found at 460
    Illegal PstI site found at 4323
    Illegal PstI site found at 7893
    Illegal PstI site found at 8070
    Illegal PstI site found at 8418
    Illegal PstI site found at 8433
    Illegal PstI site found at 9858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1356
    Illegal EcoRI site found at 3476
    Illegal EcoRI site found at 6675
    Illegal BglII site found at 5145
    Illegal BamHI site found at 7
    Illegal BamHI site found at 5814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1356
    Illegal EcoRI site found at 3476
    Illegal EcoRI site found at 6675
    Illegal PstI site found at 4323
    Illegal PstI site found at 7893
    Illegal PstI site found at 8070
    Illegal PstI site found at 8418
    Illegal PstI site found at 8433
    Illegal PstI site found at 9858
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1356
    Illegal EcoRI site found at 3476
    Illegal EcoRI site found at 6675
    Illegal PstI site found at 4323
    Illegal PstI site found at 7893
    Illegal PstI site found at 8070
    Illegal PstI site found at 8418
    Illegal PstI site found at 8433
    Illegal PstI site found at 9858
    Illegal NgoMIV site found at 4370
    Illegal NgoMIV site found at 5639
    Illegal NgoMIV site found at 5699
    Illegal NgoMIV site found at 8723
    Illegal NgoMIV site found at 9429
    Illegal NgoMIV site found at 9543
    Illegal NgoMIV site found at 9603
    Illegal AgeI site found at 4967
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10586
    Illegal SapI.rc site found at 2947
    Illegal SapI.rc site found at 5740
    Illegal SapI.rc site found at 6146
    Illegal SapI.rc site found at 10032


Design Notes

Source

All CDS designed for the operation of the Kil-Switch adapted to Chlamydomonas reinhardtii come from part collection of the iGEM Sorbonne_U 2020 team. All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).

References

1. Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

2. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.