Regulatory

Part:BBa_K337054:Design

Designed by: Rebecca Berrens   Group: iGEM10_Heidelberg   (2010-10-26)


synthetic binding site of shRNA miRhaat (imperfect) KD:28%


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to lead to a knockdown percentage of 30% if introduced in the 3'UTR of your GOI. The synthetic binding side has to be co-transfected with the corresponding synthetic miRNA.


Source

synthetic

References