Coding

Part:BBa_K3370001:Design

Designed by: CHIH-LU CHIANG   Group: iGEM20_NCTU_Formosa   (2020-10-25)


Harmonized Gloeobacter rhodopsin (GR) with linker and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1571


Design Notes

Source

Originally derived from Gloeobacter violaceus but codon harmonized for E. coli. [Nakamura et al, 2003, "Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids"].

References

  1. Kim, H.-J., et al. (2012). "An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production." Applied microbiology and biotechnology 94(4): 1079-1086
  2. Schlegel, S., et al. (2012). "Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21 (DE3)." Journal of molecular biology 423(4): 648-659.
  3. Chen, L.-C., et al. (2020). "Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use." Biosensors and Bioelectronics 155: 112111.
  4. Na, Y.-A., et al. (2015). "Growth retardation of Escherichia coli by artificial increase of intracellular ATP." Journal of industrial microbiology & biotechnology 42(6): 915-924.
  5. Kim, H. A., et al. (2017). "An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli." Microbial cell factories 16(1): 111
  6. Walter, J. M., et al. (2007). "Light-powering Escherichia coli with proteorhodopsin." Proceedings of the National Academy of Sciences 104(7): 2408-2412.
  7. Takefumi, O., et al. (2019). “X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin”
  8. GL Rosano, C., et al. (2014) “Recombinant protein expression in Escherichia coli: advances and challenges”
  9. Xiaoying, C., et al. (2013)“Fusion Protein Linkers: Property, Design and Functionality”
  10. Waldo, B. , et al. (1999) “Rapid protein-folding assay using green fluorescent protein”
  11. Que, J. , et al. (2019) “Functional Expression of Gloeobacter Rhodopsin in PSI-Less Synechocystis sp. PCC6803”
  12. Evelina.,A.,et al.(2008)”Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host”