Coding

Part:BBa_K3365052

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)


RFP

The RFP is one reporter of our system. Its excitation wavelength is 555nm and emission wavelength is 583nm in measuring its expression level by microplate spectrophotometer.

We put this part downstream of transcription unit as reporter, as we can easily know the expression level of it by detecting fluorescence intensity. dCas9 protein with subnit Omega binding to target site and subnit Omega would promote the expression of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None