RNA

Part:BBa_K3362004

Designed by: Andre Geisler   Group: iGEM20_Hamburg   (2020-10-26)


Hammerhead Ribozyme

The Hammerhead Ribozyme (HHRz) is a 5' self-cleaving ribozyme (SCRz). The first six bases are variable, because they are dependent on the bases that are downstream of the ribozyme. To modify the first six bases correctly, change them as following: Take the first six bases of the downstream part (i.e. AATTCC), create the complementary sequence (i.e. TTAAGG) and then reverse them (i.e. TTAAGG becomes GGAATT).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None