Composite

Part:BBa_K3362001

Designed by: Marvin Skiba   Group: iGEM20_Hamburg   (2020-10-25)


Target cassette

The target cassette is an engineered mechanism, that concists of amilGFP and the T500-terminator which is complementary to STAR. The binding of STAR to the T500-terminator is causing the expression of amilGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal SpeI site found at 904
    Illegal PstI site found at 918
    Illegal NotI site found at 7
    Illegal NotI site found at 911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 904
    Illegal PstI site found at 918
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None