Coding

Part:BBa_K336020:Design

Designed by: Naoto Shimada   Group: iGEM10_Tokyo_Metropolitan   (2010-10-25)


bacterial cellulose synthase B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 116
    Illegal PstI site found at 340
    Illegal PstI site found at 616
    Illegal PstI site found at 649
    Illegal PstI site found at 911
    Illegal PstI site found at 1189
    Illegal PstI site found at 1363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 116
    Illegal PstI site found at 340
    Illegal PstI site found at 616
    Illegal PstI site found at 649
    Illegal PstI site found at 911
    Illegal PstI site found at 1189
    Illegal PstI site found at 1363
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1977
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 116
    Illegal PstI site found at 340
    Illegal PstI site found at 616
    Illegal PstI site found at 649
    Illegal PstI site found at 911
    Illegal PstI site found at 1189
    Illegal PstI site found at 1363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 116
    Illegal PstI site found at 340
    Illegal PstI site found at 616
    Illegal PstI site found at 649
    Illegal PstI site found at 911
    Illegal PstI site found at 1189
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 2381
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this part has recognition sequences NgoMIV 6744- AgeⅠ 4489- BamHI 6340- PstⅠ 4479-,4703-,5012-,5274-,5552-,5726-,5864-



Source

this gene is derived from genome of A.xylimun JCM7664 strain.

References