Terminator

Part:BBa_K3351011

Designed by: Yu Zhang   Group: iGEM20_NWU-CHINA-A   (2020-09-08)


A T7 terminator of pet28a plasmid.

Summary

Terminator regions, which are located downstream of protein coding sequences, encode 3′ untranslated regions (3′-UTRs) of mRNA. Terminator regions are usually involved in two complex events: ″transcriptional termination″, which involves the cleavage of 3′-mRNA and poly(A) addition and ″post-transcriptional regulation″, in which the 3′-UTR determines the stability, translational efficiency, and localization of the mRNA. Sequence and structural cis-elements located in the 3′-UTR of mRNA transcripts interact with RNA-binding proteins (RBPs) to post-transcriptionally regulate various aspects of gene expression including mRNA localization, translation, and decay.


Reference

[1] Richard, P. and Manley, J. L. (2009) Transcription termination by nuclear RNA polymerases Genes Dev. 23, 1247– 1269
[2] Kuehner, J. N., Pearson, E. L., and Moore, C. (2011) Unravelling the means to an end: RNA polymerase II transcription termination Nat. Rev. Mol. Cell. Biol. 12, 283– 294
[3] Kuersten, S. and Goodwin, E. B. (2003) The power of the 3′ UTR: translational control and development Nat. Rev. Genet. 4, 626– 637
[4] Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., & Matsuyama, T. (2013). A genome-wide activity assessment of terminator regions in saccharomyces cerevisiae provides a "terminatome" toolbox. ACS Synthetic Biology, 2(6), 337-347.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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