Part:BBa_K3351007

P6.2, an antimicrobial peptide.

Summary

P6.2 is typical linear cation α-helical peptide.
Destroy the biomass membrane:
1. The combination of cationic peptide and anionic plasma membrane caused by electrostatic interaction.
2. AMP transforms from random coils to amphiphilic α helix, making Trp arranged on the same side of α helix; because of the hydrophobicity of AMP, Trp residues can be inserted into the carbon chain region of the membrane more easily, and AMP is arranged parallel to the membrane , With the accumulation of AMP binding, the outer layer of the bilayer lipid molecule layer expands and becomes thinner, destroying the plasma membrane.
Due to the cationic nature of P5 and P6.2, they can be inserted between negatively charged bacterial cells, interfere with the interaction of extracellular polymers (EPS), and reduce EPS accumulation.

Characterization

Figure.1 growth curve

Reference

[1] Martínez M, Polizzotto A, Flores N, Semorile L, Maffía PC. Antibacterial, anti-biofilm and in vivo activities of the antimicrobial peptides P5 and P6.2. Microb Pathog. 2020 Feb;139:103886. doi: 10.1016/j.micpath.2019.103886. Epub 2019 Nov 25. PMID: 31778756. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]