Part:BBa_K3351006

P5, an antimicrobial peptide

Summary

P5 (RIVQRIKKWLLKWWKLGY) is a synthetic cationic 18aa peptide. The outer cell membrane of gram-negative bacteria contains negatively charged lipopolysaccharide (LPS). Antimicrobial peptides can replace divalent cations such as Mg2+ and Ca2+ bound to LPS, causing the outer membrane to rupture or perforate and penetrate the outer membrane. The antimicrobial peptides penetrating the outer membrane and the negatively charged phospholipids on the inner cell membrane are combined by electrostatic attraction, and they are inserted into the phospholipid layer through hydrophobic interaction, forming a cavity or temporary channel on the cell membrane, causing the cell membrane to collapse (felt model) or making it antibacterial The peptide penetrates the cell membrane (barrel plate model).

Characterization

Figure.1 growth curve

Reference

[1] Martínez M, Polizzotto A, Flores N, Semorile L, Maffía PC. Antibacterial, anti-biofilm and in vivo activities of the antimicrobial peptides P5 and P6.2. Microb Pathog. 2020 Feb;139:103886. doi: 10.1016/j.micpath.2019.103886. Epub 2019 Nov 25. PMID: 31778756. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]