Coding

Part:BBa_K3347010:Design

Designed by: Jackson Duke Harris   Group: iGEM20_USAFA   (2020-10-16)


Dehalogenase type 2, variant 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DeHa2.1.gene sequence converted to peptide sequence and snapgene reverse transcription tool used to codon-optimize for E. coli. No illegal sites required removal for RFC 10 compatibility.



Source

DeHa1.1.gene sequence: BBa_K3347003

References