Composite

Part:BBa_K3338019

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


IL-6 Pmut-hGLuc

This part consists of an interleukin-6 promoter that is mutagenized to make it compatible with the RFC10 assembly standard that controls the expression of the reporter hGLuc. It can be used to determine the basal promoter activity and the activity following changes in the surrounding of the cell like LPS supplementation. As a control CMV-hGLuc (BBa_K3338017) can be used.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Characterization

This part was used to assess the LPS sensitivity of the mutated IL-6 promoter. It could be shown that the promoter is not induced following LPS-treatment of the transfected cells. A detailed documentation can be found on the page of the basic part BBa_K3338005.


[edit]
Categories
Parameters
None