Composite

Part:BBa_K3338015

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


CMV-EGFP-MagA-IRES-hGLuc

The composite part described here exhibits an EGFP-MagA-IRES-hGLuc-cassette under the control of a CMV-enhancer/promoter for expression in mammalian cells. It can be used to determine the expression of MagA and hGLuc at the same time from one promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2553
    Illegal BsaI.rc site found at 1885
    Illegal BsaI.rc site found at 2434
    Illegal SapI site found at 1575


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Categories
Parameters
None