Coding

Part:BBa_K3338003:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-22)


P2A self-cleaving peptide without GSG-linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part does not contain the N-terminal linker peptide "GSG" that is originally found in the porcine teschovirus-1.

Source

The sequnece was purchased from Addgene (plamsid #45350) (Potorac et al. 2016).

References

Potorac, I., Rivero-Müller, A., Trehan, A., Kiełbus, M., Jozwiak, K., Pralong, F., Hafidi, A., Thiry, A., Ménagé, J. J., Huhtaniemi, I., Beckers, A., & Daly, A. F. (2016). A vital region for human glycoprotein hormone trafficking revealed by an LHB mutation. The Journal of endocrinology, 231(3), 197–207.