Composite

Part:BBa_K3332076:Design

Designed by: Meihao Ou   Group: iGEM20_XMU-China   (2020-10-17)


J23101-OmpA 5'UTR-phnJ 0.94-Hfq binding sequence-J61048


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The OmpA 5-UTR significantly increases the half-life of the siRNA, the Hfq binding sequence protects the siRNA and strengthens the bond to the target sequence. The number 0.94 means the silence probability (up to 1.0).


Source

Construct from existed parts according to Standard BioBrick Assembly


References