Composite

Part:BBa_K3332070:Design

Designed by: Siru Zhou   Group: iGEM20_XMU-China   (2020-10-17)


J23100-RBS-phnJ_mut40&16-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
    Illegal AgeI site found at 329
    Illegal AgeI site found at 836
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Replace Arg40 with Phe40， replace Thr16 with Ser16


Source

Construct from existed parts according to Standard BioBrick Assembly

References