Part:BBa_K3332000:Design

iNAP-his-tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 272
  Illegal XhoI site found at 298
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 38
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The iNAP sequence we use is different from what we mentioned in this part. If you have any questions about it, please contact with us via email igem_xmu@163.com.

Source

Circularly permuted YFP(cpYFP)and Rex from Thermus aquaticus(T-Rex). We ordered the part DNA from Genscript® company along with CDS below.