Part:BBa_K3331011:Design

B.S galU+P.A pgmA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1557
  Illegal BglII site found at 1590
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 912
  Illegal AgeI site found at 1146
  Illegal AgeI site found at 1785
  Illegal AgeI site found at 2163
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We optimized the sequence with the codon preferred by Bacillus subtilis

Source

The part galU is from Bacillus subtilis subsp. subtilis str. 168. The part pgmA is from Pseudomonas aeruginosa PAO1.