Coding
pgmA

Part:BBa_K3331003

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-20)


B.S. pgmA

pgmA [Bacillus subtilis (strain 168)]

Encodes 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. This enzyme catalyzes the reversible reaction between 2-phosphoglycerate and 3-phosphoglycerate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1875bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
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Categories
Parameters
None