Composite

Part:BBa_K3329068:Design

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-spider fibroin*2-sfGFP for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2041
    Illegal NotI site found at 635
    Illegal NotI site found at 1700
    Illegal NotI site found at 1772
    Illegal NotI site found at 1790
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2453
    Illegal XhoI site found at 1979
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1936
    Illegal AgeI site found at 2932
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E. coli


Source

From Article///

References