Composite

Part:BBa_K3329062:Design

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-20)


SpyTag003*3-Scl2*2-sfGFP for P. Pastoris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 787
    Illegal NgoMIV site found at 1116
    Illegal NgoMIV site found at 1663
    Illegal NgoMIV site found at 1695
    Illegal AgeI site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862
    Illegal BsaI.rc site found at 1588


Design Notes

Optimized for P. Pastoris


Source

From Article///

References