Translational_Unit
Part:BBa_K332033:Design
Designed by: KUO, CHIA-CHUN Group: iGEM10_NCTU_Formosa (2010-10-24)
We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein.
Therefore, we took advantage of the interaction between 37° C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit.
The following is a description of our prescribed strategy.
First, we use the constitutive promoter, 37° C induced RBS (K115002), the tetR gene and terminators. We will call this part the “Regulator”.