Coding

Part:BBa_K3320013:Design

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


GFPmut3 + HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

GFP derived from Aequeora victoria with secretion tag from E. coli's alpha-hemolysin system.

References

Thomas, S., Holland, I. B. & Schmitt, L. The Type 1 secretion pathway - The hemolysin system and beyond. Biochimica et Biophysica Acta - Molecular Cell Research 1843, 1629–1641 (2014).

Gentschev, I., Dietrich, G. & Goebel, W. The E. coli α-hemolysin secretion system and its use in vaccine development. Trends in Microbiology 10, 39–45 (2002).