Composite

Part:BBa_K3317031:Design

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)


BMC shell proteins ABJKN from S. enterica LT2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 41
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1761
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 1388
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

WT sequences of S. enterica LT2 (NC_003197.1) contain illegal sites SpeI in 2292 and XbaI in 41

Source

described by Yung, M. C et. to 2017

References

Yung, M. C., Bourguet, F. A., Carpenter, T. S., & Coleman, M. A. (2017). Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. Microbial cell factories, 16(1), 71.