Composite
Part:BBa_K3317031:Design
Designed by: erick hernandez Group: iGEM19_UANL (2019-10-18)
BMC shell proteins ABJKN from S. enterica LT2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 41
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1761
Illegal BamHI site found at 1900 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 41
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 41
Illegal NgoMIV site found at 1388
Illegal AgeI site found at 1655
Illegal AgeI site found at 1691 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
WT sequences of S. enterica LT2 (NC_003197.1) contain illegal sites SpeI in 2292 and XbaI in 41
Source
described by Yung, M. C et. to 2017
References
Yung, M. C., Bourguet, F. A., Carpenter, T. S., & Coleman, M. A. (2017). Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. Microbial cell factories, 16(1), 71.