Coding

Part:BBa_K3317001:Design

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)


Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
    Illegal NgoMIV site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372


Design Notes

sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided, a signal peptide () was added at its C terminal end together with a flexible G4S linker


Source

From Sphingobium sp. SYK-6 ACCESSION BAK65949

References

Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.

Wagner, H. J., Capitain, C. C., Richter, K., Nessling, M., & Mampel, J. (2017). Engineering bacterial microcompartments with heterologous enzyme cargos. Engineering in Life Sciences, 17(1), 36-46.