Composite

Part:BBa_K3316004:Design

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


SpyCatcher-CsgA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To help purify the fusion protein, we added a 6-His tag at the N-terminus of the protein. The choice of linker is to minimize constraints in conformation in either portion of the fusion protein product due to sterics.


Source

The CsgA sequence was derived from the Escherichia coli K12 genome. The SpyCatcher moiety is endogenous to Streptococcus pyogenes.

References