Device
Part:BBa_K331000:Design
Designed by: Lisza Bruder Group: iGEM10_Lethbridge (2010-06-28)
IPTG inducible Lumazine Synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We followed Seebeck et al. and mutated Arg83, Thr 86, Thr 120, and Gln123 to glutamates
Source
Sequence from Auquifex aeolicus
References
Seebeck, F. P., Woycechowsky, K. J., Zhuang, W., Rabe, J. P., and Hilvert, D., (2006). A simple tagging system for protein encapulation. J. Am. Chem. Soc. 128, 4516-4517