Coding
MBP

Part:BBa_K3308090:Design

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)


Maltose binding protein (malE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.



Source

The source of this composite part was sequences of Basic parts found through Genbank.

References