Coding
N1-E

Part:BBa_K3308065:Design

Designed by: University of Pittsburgh 2019 iGEM Team: Jemy Varghese, Harrison Green, Ripal Sheth, Victor So, Mel Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)


GB1-GTNPC- n side extein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 43


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.


Source

The source of this composite part was sequences of Basic parts found through Genbank.


References