Coding
gpD+GB1

Part:BBa_K3308060:Design

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)


Full spliced product- gpD-GIGGG-SICST-GB1 stop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.



Source

The source of this composite part was sequences of Basic parts found through Genbank.

References