Coding
IMPDH-N2/2

Part:BBa_K3308047:Design

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-05)


Nested IMPDH N terminus- [IMPDH-1 N (56-101)] + stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The split site of this half of IMPDH intein was strategically chosen to satisfy the biochemical requisites need for the functional splicing of NpuDnaE.


Source

The source of the intein sequence and characterization data is InBase.

References