Composite

Part:BBa_K3301003:Design

Designed by: Henry G Ryle   Group: iGEM19_Baltimore_BioCrew   (2019-10-15)


EndoA Cell Growth Inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made two major changes. One was replacing the intial terminator to lower complexity, and the other was to cause the toxin to be secreted when away from xylose, rather than in its presence.


Source

This part was initially used by the HKUST team, and due to the success they had with it, we decided to use it in our project.

References