Coding
Part:BBa_K3301001:Design
Designed by: Maria Lyons, Hailey Ho, Vina Chen, Zoe Hsieh, Josh Dayie Group: iGEM19_Baltimore_BioCrew (2019-10-15)
Codon optimized B. theta gene BT2268
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1735
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1178
Illegal BsaI.rc site found at 1634
Illegal SapI site found at 1553
Design Notes
Removed initial signal sequence. Codon optimized for E. coli.
Source
Synthesized by IDT