Part:BBa_K3298001

iGEM

This part is DNA sequence encoded a secret message. This sequence can be translated into a senstence: IGEM!SHANGHAI_CITY IS COMING!

Usage and Biology

We added ture primer binding site at the end of sequence.

We stored the DNA message on the paper. After dropped on the paper, the DNA solution will evaporate and DNA inside which is kept on the paper. As it is hard to test out whether or not there is DNA on a certain paper, this method for storing DNA is safe. What’s more, paper is cheap and normal, which means everyone can get and carry it easily. Additionally, DNA has a high solubility, which means that as long as recipients immerse the paper they get into water, the DNA can solve into it and become solution state again, which is easy for the recipients to extract and test.

Our target is to find out whether storing DNA on paper is practical and which kind of paper, which way to store the DNA can have the best effect.

Experimental group:

Experimental results:

From the results of gel running, it can be seen in figure 2 that groups 3,6 and 7, namely, stored DNA best in paper jam (white), IGEM sticker and solution.

The PCR fragment sequencing result

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 42
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]