Project

Part:BBa_K3297000:Design

Designed by: Tyler Schraeder   Group: iGEM19_Mines   (2019-10-20)


Cadmium binding system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 649
    Illegal BamHI site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 347


Design Notes

BamHI cut sight was added for directional cloning of the promoter sequence. Some silent mutations to the maltose binding sequence were made in order to satisfy complexity, and restriction site requirements.


Source

The binding protien was studied by Dr. Mehran Pazirandeh, Dr. Bridget Wells, and Dr. Rebecca Ryan from the Center for Bio/Molecular Science and Engineering at the Naval Research Laboratory.

References