Regulatory

Part:BBa_K3292005:Design

Designed by: Gerardo David Díaz Renovato, Abril Diosdado Hernández   Group: iGEM19_Tec-Monterrey   (2019-10-20)


DNA synthesis regulated promoter (yorB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design considerations already appear in the description


Source

We obtained the sequence of this part from the NCBI genome database using the primers provided by Urban et al. (2007)

References

Hutter, B., Fischer, C., Jacobi, A., Schaab, C., & Loferer, H. (2004). Panel of Bacillus subtilis reporter strains indicative of various modes of action. Antimicrobial agents and chemotherapy, 48(7), 2588–2594. doi:10.1128/AAC.48.7.2588-2594.2004

Urban, A., Eckermann, S., Fast, B., Metzger, S., Gehling, M., Ziegelbauer, K., Rübsamen-Waigmann, H., Freiberg, C. (2007). Novel whole-cell antibiotic biosensors for compound discovery. Applied and environmental microbiology, 73(20), 6436–6443. doi:10.1128/AEM.00586-07