Project

Part:BBa_K329069:Design

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-27)


Population counter Tetracycline resistant after recombination - PmS-MTet-LuxI


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2876
    Illegal BamHI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1337
    Illegal NgoMIV site found at 1705
    Illegal NgoMIV site found at 1865
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.


Source

2010 Distribution kit and Mazel Lab.

References