Translational_Unit

Part:BBa_K3288054

Designed by: Jason Park   Group: iGEM19_SIS_Korea   (2019-10-16)


light inducible expression of mScarlet

We used pDawn plasmid DNA to induce the expression of PET degradation enzymes with light. The mScarlet coding sequence was inserted into pDawn by using the general molecular cloning technique.


Usage and Biology

236px-PDawn-mScarlet.png

The E. coli BL21 with the transformed pDawn-mScarlet was cultured in LB(with Kanamycin). After it was cultured in LB (with Kanamycin) diluted 1/100, for 2 hours 30 mins at 37 ℃. Then, we induced mScarlet expression by lighting it with white LED.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
    Illegal XhoI site found at 4151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI site found at 3660
    Illegal BsaI.rc site found at 525


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Parameters
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