Coding

Part:BBa_K3288013:Design

Designed by: Michelle Ahn   Group: iGEM19_SIS_Korea   (2019-10-15)


signal peptide (NSP4) fused PETase(28-290;S121E/D186H/R280A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 850
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Expression unit combining CP44 promoter and ribosome binding site derived from the pDawn vector for continuous expression of target genes in E. coli


Source

This part was synthesized from Twist Bioscience.

References