Other
Part:BBa_K3286267:Design
Designed by: Marijn Ceelen Group: iGEM19_Wageningen_UR (2019-10-21)
phage Lambda gpD insertion homology region 2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The length of the homology region was balanced between ease of use and recombination efficiency. We believe that 200 bp is a good compromise.
Source
The homology region has been amplified from the phage Lambda genome using PCR.